PUBLISHED WORK
2024
Probing electrostatic and hydrophobic associative interactions in cells
W Zuo, MR Huang, F Schmitz, AJ Boersma
The Journal Physical Chemistry B doi.org/10.1021/acs.jpcb.4c05990
SA Mersch, C McCue, A Aristidou, ED Sheets, AJ Boersma, AA Heikal
Physical Chemistry Chemical Physics doi.org/10.1039/D4CP03850A
DNA and mRNA as molecular speed bumps in Escherichia coli's cytoplasm [New & Notable]
AJ Boersma
Biophysical Journal doi.org/10.1016/j.bpj.2024.04.012
B Monterroso, W Margolin, AJ Boersma, G Rivas, B Poolman, S Zorrilla
Chemical Reviews doi.org/10.1021/acs.chemrev.3c00622
SA Mersch, S Bergman, ED Sheets, AJ Boersma, AA Heikal
Physical Chemistry Chemical Physics doi: 10.1039/D3CP04733D.
2023
A physicochemical perspective on cellular ageing
SN Mouton, AJ Boersma, LM Veenhoff
Trends in Biochemical Sciences doi: 10.1016/j.tibs.2023.08.007. Online ahead of print.
Genetically-encoded probes to determine nonspecific hydrophobic and electrostatic binding in cells
W Zuo, MR Huang, F Schmitz, AJ Boersma
bioRxiv doi: https://doi.org/10.1101/2023.06.27.546658
Microgels As Platforms for Antibody-mediated Cytokine Scavenging
S Boesveld, Y Kittel, Y Luo, A Jans, B Oezcifci, M Bartneck, C Preisinger, D Rommel, T Haraszti, SP Centeno, AJ Boersma, L De Laporte, C Trautwein, AJC Kuehne, P Strnad
Advanced Healthcare Materials 2023: e2300695. Online ahead of print.
T Pittas, AJ Boersma
bioRxiv doi: https://doi.org/10.1101/2023.02.23.529735
Cell wall damage increases macromolecular crowding effects in the Escherichia coli cytoplasm
T Pittas, W Zuo, AJ Boersma
iScience 2023 DOI: https://doi.org/10.1016/j.isci.2023.106367
2022
High macromolecular crowding in liposomes from microfluidics
LPB Guerzoni, AVC de Goes, M Kalacheva, J Hadula, M Mork, L De Laporte, AJ Boersma
Advanced Science 9 (27), 2201169
A FRET-based method for monitoring structural transitions in protein self-organization
Q Wan, SN Mouton, LM Veenhoff, AJ Boersma
Cell Reports Methods 2 (3), 100184
2021
Kay TM, Aplin CP, Simonet R, Beenken J, Miller RC, Libal C, Boersma AJ, Sheets ED, Heikal AA.
Front Mol Biosci. 2021 p. 878
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound.
Zhao P, Huo S, Fan J, Chen J, Kiessling F, Boersma AJ, Göstl R, Herrmann A.
Angew Chem Int Ed Engl. 2021 May 3. doi: 10.1002/anie.202105404. Online ahead of print.
Fluorescence Depolarization Dynamics of Ionic Strength Sensors using Time-Resolved Anisotropy.
Aplin CP, Miller RC, Kay TM, Heikal AA, Boersma AJ, Sheets ED.
Biophys J. 2021 Feb 11:S0006-3495(21)00138-7. doi: 10.1016/j.bpj.2021.01.035. Online ahead of print.
Engineering Crowding Sensitivity into Protein Linkers.
Pittas T, Zuo W, Boersma AJ.
Methods Enzymol. 2021 doi: 10.1016/bs.mie.2020.09.007
Controlling Optical and Catalytic Activity of Genetically Engineered Proteins by Ultrasound.
Zhou Y, Huo S, Loznik M, Göstl R, Boersma AJ, Herrmann A.
Angew Chem Int Ed Engl. 2021 Oct 26. doi: 10.1002/anie.202010324. Online ahead of print.
2020
Mouton SN, Thaller DJ, Crane MM, Rempel IL, Terpstra OT, Steen A, Kaeberlein M, Lusk CP, Boersma AJ, Veenhoff LM.
Elife. 2020 Sep 29;9:e54707. doi: 10.7554/eLife.54707.
Mouton SN, Veenhoff LM, Boersma AJ.
Methods Mol Biol. 2020; 2175:169-180. doi: 10.1007/978-1-0716-0763-3_12.
Löwe M, Kalacheva M, Boersma AJ, Kedrov A.
FEBS J. 2020 May 28. doi: 10.1111/febs.15429. Online ahead of print.
Modular and Versatile trans-Encoded Genetic Switches.
Paul A, Warszawik EM, Loznik M, Boersma AJ, Herrmann A
Angew Chem Int Ed Engl. 2020 Apr 30. doi: 10.1002/anie.202001372. Online ahead of print.
Miller RC, Aplin CP, Kay TM, Leighton R, Libal C, Simonet R, Cembran A, Heikal AA, Boersma AJ, Sheets ED
J Phys Chem B. 2020 Apr 30;124(17):3447-3458. doi: 10.1021/acs.jpcb.9b10498. Epub 2020 Apr 16.
Supercharged Proteins and Polypeptides.
Ma C, Malessa A, Boersma AJ, Liu K, Herrmann A.
Adv Mater. 2020 Jan 15:e1905309. doi: 10.1002/adma.201905309
This article is part of the Special Issue: From Responsive Materials to Interactive Materials
2019
Syga Ł, de Vries RH, van Oosterhout H, Bartelds R, Boersma AJ, Roelfes G, Poolman B.
Chembiochem. 2019 Dec 9. doi: 10.1002/cbic.201900655.
DNA Nanotechnology Enters Cell Membranes.
Huo S, Li H, Boersma AJ, Herrmann A.
Adv Sci (Weinh). 2019 Mar 20;6(10):1900043. doi: 10.1002/advs.201900043
Highlighted in Advanced Science News, April 2019.
This article is part of the Advanced Science 5th anniversary interdisciplinary article series.
Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress.
Liu B, Hasrat Z, Poolman B, Boersma AJ.
J Bacteriol. 2019 Apr 24;201(10). pii: e00708-18. doi: 10.1128/JB.00708-18
Selected as Article of Significant Interest (spotlight) in J. Bacteriol.
Schwarz J, J Leopold H, Leighton R, Miller RC, Aplin CP, Boersma AJ, Heikal AA, Sheets ED.
Methods Appl Fluoresc. 2019 Feb 19;7(2):025002. doi: 10.1088/2050-6120/ab0242
Leopold HJ, Leighton R, Schwarz J, Boersma AJ, Sheets ED, Heikal AA.
J Phys Chem B. 2019 Jan 17;123(2):379-393. doi: 10.1021/acs.jpcb.8b09829
2018
How Important Is Protein Diffusion in Prokaryotes?
Schavemaker PE, Boersma AJ, Poolman B.
Front Mol Biosci. 2018 Nov 13;5:93. doi: 10.3389/fmolb.2018.00093.
Special issue on biochemical reactions in cytomimetic media edited by A.P. Minton and G. Rivas
Lee HB, Cong A, Leopold H, Currie M, Boersma AJ, Sheets ED, Heikal AA.
Phys Chem Chem Phys. 2018 Oct 7;20(37):24045-24057. doi: 10.1039/c8cp03873b
Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells.
Liu B, Mavrova SN, van den Berg J, Kristensen SK, Mantovanelli L, Veenhoff LM, Poolman B, Boersma AJ.
ACS Sens. 2018 Sep 28;3(9):1735-1742. doi: 10.1021/acssensors.8b00473
Selected in "Rising Stars in Sensing" virtual issue of ACS Sensors 2020
Höfig H, Otten J, Steffen V, Pohl M, Boersma AJ, Fitter J.
ACS Sens. 2018 Aug 24;3(8):1462-1470. doi: 10.1021/acssensors.8b00143
2017
Ionic Strength Sensing in Living Cells.
Liu B, Poolman B, Boersma AJ.
ACS Chem Biol. 2017 Oct 20;12(10):2510-2514. doi: 10.1021/acschembio.7b00348
Highlighted in Chemical & Engineering News, October 2017
Cover image ACS Chem. Biol. October 2017
Meet our authors highlight Boqun Liu
Fluorescence Dynamics of a FRET Probe Designed for Crowding Studies.
Currie M, Leopold H, Schwarz J, Boersma AJ, Sheets ED, Heikal AA.
J Phys Chem B. 2017 Jun 15;121(23):5688-5698. doi: 10.1021/acs.jpcb.7b01306.
Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding.
Liu B, Åberg C, van Eerden FJ, Marrink SJ, Poolman B, Boersma AJ.
Biophys J. 2017 May 9;112(9):1929-1939. doi: 10.1016/j.bpj.2017.04.004.
Microorganisms maintain crowding homeostasis.
van den Berg J, Boersma AJ, Poolman B.
Nat Rev Microbiol. 2017 May;15(5):309-318. doi: 10.1038/nrmicro.2017.17
2016
Semisynthetic Nanoreactor for Reversible Single-Molecule Covalent Chemistry.
Lee J, Boersma AJ, Boudreau MA, Cheley S, Daltrop O, Li J, Tamagaki H, Bayley H.
ACS Nano. 2016 Sep 27;10(9):8843-50. doi: 10.1021/acsnano.6b04663
Highlighted in Nanotechweb.org, 2016.
2015
Associative Interactions in Crowded Solutions of Biopolymers Counteract Depletion Effects.
Groen J, Foschepoth D, te Brinke E, Boersma AJ, Imamura H, Rivas G, Heus HA, Huck WT.
J Am Chem Soc. 2015 Oct 14;137(40):13041-8. doi: 10.1021/jacs.5b07898
Highlighted in Derek Lowe’s “in the pipeline” blog
Protein engineering: The power of four.
Boersma AJ, Roelfes G.
Nat Chem. 2015 Apr;7(4):277-9. doi: 10.1038/nchem.2220
A sensor for quantification of macromolecular crowding in living cells.
Boersma AJ, Zuhorn IS, Poolman B.
Nat Methods. 2015 Mar;12(3):227-9, 1 p following 229. doi: 10.1038/nmeth.3257
Highlighted in NWO newsletter, 2015
Highlighted in Biophysical Society newsletter (with interview), 2015
News articles in kennislink.nl, University of Groningen website, AzoSensors.com, Science Newsline, Phys.org, Technobahn, EurekAlert!, Science Daily, Bioportfolio, Nanowerk Nanotechnology News, and others.
Draksharapu A, Boersma AJ, Browne WR, Roelfes G.
Dalton Trans. 2015 Feb 28;44(8):3656-63. doi: 10.1039/c4dt02734e.
Draksharapu A, Boersma AJ, Leising M, Meetsma A, Browne WR, Roelfes G.
Dalton Trans. 2015 Feb 28;44(8):3647-55. doi: 10.1039/c4dt02733g.
EARLIER
Continuous stochastic detection of amino acid enantiomers with a protein nanopore.
Boersma, A.J.; Bayley, H. Angew. Chem. Int. Ed. 2012, 51, 9606.
Real-time detection of multiple neurotransmitters with a protein nanopore.
Boersma, A.J.; Brain, K.L.; Bayley, H. ACS Nano 2012, 6, 5304.
Highlighted in Nanomedicine, 2012.
Ligand denticity controls enantiomeric preference in DNA-based asymmetric catalysis.
Boersma, A.J.; de Bruin, B.; Feringa, B.L.; Roelfes, G. Chem. Commun. 2012, 2394.
Highlighted in Chemistry & Industry, 2012.
Catalytic enantioselective syn hydration of enones in water using a DNA-based catalyst.
Boersma, A.J.; Coquière, D.; Geerdink, D.; Rosati, F.; Feringa, B.L.; Roelfes, G. Nature Chem. 2010, 2, 991.
Highlighted on the NWO website, Chemisch2Weekblad
Synfacts highlight of the month February
Selected in Faculty of 1000
On the role of DNA in DNA-based catalytic enantioselective conjugate addition reactions.
Dijk, E.W.; Boersma, A.J.; Feringa, B.L.; Roelfes, G. Org. Biomol. Chem. 2010, 8, 3868.
DNA-based asymmetric catalysis.
Boersma, A.J.; Megens, R.P.; Feringa, B.L.; Roelfes, G. Chem. Soc. Rev. 2010, 39, 2083.
A kinetic and structural investigation of DNA-based asymmetric catalysis using the first generation ligands.
Rosati, F.; Boersma, A.J.; Klijn, J.E.; Meetsma, A.; Feringa, B.L.; Roelfes, G. Chem. Eur. J. 2009, 15, 9596.
Enantioselective Friedel-Crafts reactions in water using a DNA-based catalyst.
Boersma, A.J.; Feringa, B.L.; Roelfes, G. Angew. Chem. Int. Ed. 2009, 48, 3346.
Highlighted in Synfacts 2009(7)
DNA-based asymmetric catalysis: Sequence-dependent rate acceleration and enantioselectivity.
Boersma, A.J.; Klijn, J.E.; Feringa, B.L.; Roelfes, G. J. Am. Chem. Soc. 2008, 130, 11783.
Highlighted in JACS Select #4
α,β-Unsaturated 2-acyl imidazoles as a practical class of dienophiles for the DNA-based catalytic asymmetric Diels-Alder reaction in water.
Boersma, A.J.; Feringa, B.L.; Roelfes, G. Org. Lett. 2007, 9, 3647.
Highlighted on organic-chemistry.org, august 2008
Highly enantioselective DNA-based catalysis.
Roelfes, G.; Boersma, A.J.; Feringa, B.L. Chem. Commun. 2006, 635.
Hot paper in Chemical Communications.
Highlighted in Chemistry World, march 2006
Highlighted in Nature news & views, 7 dec 2006
Metal-catalyzed cotrimerization of arynes and alkenes.
Quintana, I.; Boersma, A.J.; Peña, D.; Pérez, D.; Guitián, E. Org. Lett. 2006, 8, 3347.
Catalytic enantioselective conjugate addition of dialkylzinc reagents to N-substituted-2,3-dehydro-4-piperidones.
Sebesta, R.; Pizzuti, M.G.; Boersma, A.J.; Minnaard, A.J.; Feringa, B.L. Chem. Commun. 2005, 1711.